Evolution of DNA - First Schism

First Protein Transcription
First Genetic Replication
First Feedback
Puddle Evolution
First Dispersal & Evolution
First Parasite
First Organism
First Cell Metabolism
First Self-Sufficiency
Aromatic Assistants
First Assimilation
First Transfer Molecules
Eight Molecule Life
Complementary Base Pairs
Energy Sources
Conquering the Oceans
First Cells
Cellular Explosion
Gene Regulation
First DNA
Wider Reading Frames
Complementary Triplets
Cellular Scripts
The Spread of Foxy
Second Parasite-- Transposons
First Schism
Improved Gene Regulation
Cell Structures
Eukaryote Explosion
Multi-Cellular Scripts
Cambrian Explosion
Appendix 1-- Prebiotic Earth
Appendix 2-- Primordial Puddles
Appendix 3-- Primordial Catalysts
Appendix 4-- C Value Enigma
Cast of Characters

According to current theory, there are three major domains of living organisms-- the Prokaryotes (bacteria and cyanobacteria), the Archaea (thermophiles, halophiles etc) and the Eukaryotes (protozoa, fungi, plants and animals).

All three domains use DNA for coding genetic material. In Prokaryotes and Archaeans it's usually in the form of a single loop, while Eukaryotes usually have multiple chromosomes, with the DNA bound into complex structures wrapped around polymers of histone (an amino acid).


Evolutionary geneticists sometimes talk about LUCA, the Last Universal Common Ancestor (sometimes also called a progenote). This is the theoretical early organism that existed just before the development of the three major life domains.

The search for LUCA is fraught with difficulties, especially because modern organisms are very willing to swap genes between different species (called 'horizontal transfer'), and that process was probably even more common, back when organisms were simpler. However, from our evolutionary viewpoint, this is about where LUCA happens.

Many bacteria contain moderate quantities of microsatellite DNA , so it seems likely that at least some Foxy function developed before the separation of early life forms into the three domains. But there are major divergences between the domains in many important areas, so it seems likely that the schism occurred before cells had specified important structures such as flagella.

Many microbiologists are examining small chemical clues, to evaluate the relationship between the different life domains, and their probable evolutionary paths. We won't be able to add any new information to that search, but let's at least take a brief look at the three life domains, and how they probably were related to the introduction of Foxy.


The guiding philosophy for bacteria and cyanobacteria (blue-green algae) is KISS-- Keep it Simple, Stupid. They are small cells with a simple genome, and their cell interior doesn't have very much structure. For example, the DNA in most bacterial cells is not organized into a separate nucleus, and they lack most of the organelles that are found in the cells of their more complex cousins.

It's a successful strategy, and bacteria are found in just about every nook and cranny of Earth. In fact, the typical human body contains ten times as many bacterial cells, as human ones!

Members of this domain tend to reproduce quickly, and for the most part, they strive for the absolute minimum of genetic content and the simplest of cell metabolisms. Anything that can shave a few minutes off the cell generation time is a good thing, from a bacterial point of view. And they've had trillions of cell generations in which to perfect the process.

LUCA may have been similar to a bacterial cell, or even simpler. But it's also possible that it was more complexly structured than modern bacteria. In the latter case, bacteria would have gradually substituted simpler systems with fewer parts. You might think of a bacteria as a race car that started out as a sedate family sedan, but gradually dispensed with amenities such as doors, cup holders, turn signals and air conditioning. All that's left is speed and fuel efficiency.

Many species of bacteria contain transposons, but this domain includes relatively few self-splicing introns. That suggests that bacteria may have dispensed with most helper chains and scripts, and concentrated mainly on efficient proteins to manage cell metabolism.

Once a protein is transcribed, it can fold up into its final structure and start working immediately. So it appears that many bacteria don't want to wait around for some leisurely script to be run, and want to get right to business ASAP pronto.


The guiding philosophy for the Archaea is BUSH-- Build Us Sturdy, Hercules. Many of the Archaea live in extreme environments, where heat, cold, extreme acidity or other hazards make it impossible for bacteria to live.

The Archaea have some chemical modifications that make them more tolerant of extreme conditions. For example, the lipids in their cell membranes are connected by a ether bond rather than a flimsier ester bond, and the lipids themselves are tougher (they are built with the same chemical system that produces rubber).

The Archaea seem midway between the bacteria and the eukaryotes, so it's possible that they are the closest to LUCA.

About 3.7 billion years ago, the Earth and Moon suffered an extremely intense period of large meteor impacts known as the Late Heavy Bombardment. It's possible that the only organisms to survive the resulting chaos and climatic change were deep-soil and extreme-condition cells such as the Archaea, which would explain why they are now closest to the original roots of life.

On the other hand, it's also possible that the Archaea may also have 'stripped down' over the past few billion years, and they may actually be simpler than LUCA. As with the bacteria, their selective pressure has been for simplicity and reliability, and they may have evolved into efficient systems to replace the fancy-pants scripts of their ancestors.

Perhaps the best analogy for the Archaea would be the Demolition Derby car, that started out as a sedate family sedan, but then lost its window glass and flammable interior details, and traded comfort for extra reinforced welding, instead.


The guiding philosophy for the protozoa and multi-cellular organisms is MICE-- Make It Complicated, Einstein. We eukaryotes have complex cells, with a separate cell nucleus, and many advanced cell structures. Members of this life domain take longer to grow and reproduce, but they can also grow to larger sizes, and have a much wider repertoire of metabolic tricks.

For example, one of the simplest of eukaryotes is the common brewer's yeast, Saccharomyces cerevisiae. People tend to think of it as just another microorganism, but it's much bigger than the typical bacterial cell (3 to 5 microns vs .5 to 1 micron), and it contains much more DNA (12 million base pairs, vs about 4 million base pairs in a typical bacteria). Yeast cells are much more complex than bacteria-- they include a separate nucleus, and many specialized cell structures. They also grow more slowly than bacteria (under ideal conditions they take about 2.6 hours to split, while some bacteria can do the same in 15 to 20 minutes).

Eukaryotes are so closely tied to Foxy scripting that in the remainder of this book, we'll only consider them. They have definitely taken scripting and gene regulation to its highest level (which is probably why the average Eukaryote gene contains about 7 introns).

You might think of Eukaryotes as luxury cars that started out as a sedate family sedan, but then let gadgetitis take hold, complete with GPS navigation, an espresso machine on the dash, and cupholders with napkin dispensers .

Foxy and the Domains

Eukaryotes are built from complex cells with a nucleus, mitochondria and other organelles. If our 'scripting' theory is correct, they would be organized with a relatively large number of scripts, which in turn would mean a relatively high percentage of repetitive DNA in introns and/or satellite DNA. In general, more complex Eukaryotes do have more repetitive DNA, though the total genome size varies enormously between different species (a problem known as the C-value enigma, which we'll cover in Appendix 4).

Prokaryote and Archaea cells also contain some repetitive DNA , though in much smaller quantities than in eukaryotes. A few genes in these simpler organisms contain introns (primarily 'self-splicing' type II introns), but most repetitive DNA occurs as transposons instead.

There are two theories for the large difference in intron counts between prokaryotes and eukaryotes-- that most introns evolved after the split between the domains (called 'intron early'), and that bacteria and other simple organisms lost their introns (called 'intron late').

Intron Early

Since Prokaryotes in particular are small organisms with rapid growth and high turnover, it may be that Prokaryote and Archaeal cells started out with a Foxy system to specify cell structures such as flagella, but then replaced most or all of the scripts with protein-based controls, later on.

Modern bacteria live, reproduce and die very quickly, and script-based structures may have simply taken too much time or resources. So, even though it is more difficult for protein-based control systems to evolve, they may have provided enough of a metabolic advantage to out-compete the use of scripts.

If that is true, then Foxy may have still provided a method for the first bacteria to evolve in prototype form, and it might still be used in a few places where a more efficient control system has never evolved, or as a secondary feature to allow prokaryote species to evolve new features more easily.

Introns Late

It's also possible that Foxy (and the intron data that it uses) only appeared in the Eukaryotes, after they had already diverged from Prokaryotes and Archaea, or that it was used in some primitive form before the first schism, and then reached its full maturity only in eukaryotes.

Eukaryote cells tend to be longer-lived than their bacterial cousins, with fewer, larger individuals. Because of that, efficient evolutionary change is more important to them, and Foxy would have been relatively more advantageous to them.

The short generation time and large population sizes in the smaller, simpler Prokaryotes and Archaea may have allowed them to accept a higher mutation rate so they could evolve via the metabolically more simple protein-based controls, rather than Foxy scripts.

Horizontal Transfers

Any efforts to track down the early genetic heritage among the different domains is complicated by 'horizontal transfers' of genetic material between one cell and another.

This kind of genetic transfer still goes on in modern bacteria, which frequently exchange genetic material with other individuals and even other species.

It seems likely that this sort of horizontal genetic transfer also occurred in early organisms (probably more frequently, since the organisms themselves were simpler and more able to use novel genes coming in from somewhere else).

Horizontal transfers of genetic information would have made the schism between the different life domains much more gradual, and it would have made evolution proceed more quickly since a useful enzyme discovered by one species would have ended up in many other species as well, without the need for them to discover it independently.